Recycling and sequential chromatography is a streamlined model of this process. Countercurrent chromatography is understood for its high dynamic range of scalability: milligram to kilogram quantities purified chemical components could also be obtained with this method. The Chemical Record. 3 (3): 133-143. doi:10.1002/tcr.10057. Chemical evaluation. New York: J. Wiley. Chromatographic Science Series Vol 82. New York: CRC Press. 38. Boston: Elsevier Science Ltd. Poppy straw is first pulverized, then washed as many as six to ten or more instances in water which can have an acid added to increase solubility, to provide poppy straw concentrate (PSC, also known as focus of poppy straw, CPS). Generally, an ionic displacer (or eluter) is added to cellular phase and an ionic retainer is added to the stationary section. In an conventional CCC experiment the biphasic solvent system is pre-equilibrated before the instrument is crammed with the stationary part and equilibrated with the mobile section.

Phase I is already full with a 2,800 seat amplified hall and a 300-seat theater for smaller productions. Sutherland, I. A.; Brown, L.; Forbes, S.; Games, G.; Hawes, D.; Hostettmann, K.; McKerrell, E. H.; Marston, A.; Wheatley, D.; Wood, P. (1998). "Countercurrent Chromatography (CCC) and its Versatile Application as an Industrial Purification & Production Process". DeAmicis, Carl; Edwards, Neil A.; Giles, Michael B.; Harris, Guy H.; Hewitson, Peter; Janaway, Lee; Ignatova, Svetlana (2011). "Comparison of preparative reversed phase liquid chromatography and countercurrent chromatography for the kilogram scale purification of crude spinetoram insecticide". Ignatova, Svetlana; Wood, Philip; Hawes, David; Janaway, Lee; Keay, David; Sutherland, Ian (2007). "Feasibility of scaling from pilot to process scale". Ian A. Sutherland (2007). "Recent progress on the industrial scale-up of counter-present chromatography". Berthod, Alain; Maryutina, Tatyana; Spivakov, Boris; Shpigun, Oleg; Sutherland, Ian A. (2009). "Countercurrent chromatography in analytical chemistry (IUPAC Technical Report)" (PDF). 2009). "Performance comparability using the GUESS mixture to judge counter-present chromatography instruments".

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